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The slices were rinsed and placed into a chamber embedded in the stage of an Olympus BX51WI microscope (Olympus) coupled to a 12-bit CCD fluorescent camera (SensiCam, PCO imaging)

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The samples’ OD was compared to a standard curve (range from 3.906 to 250 pg/mL) using a plate reader (Infinite 200 Pro MPlex, Tecan, Männedorf, Switzerland) set to read the plate at 450 nm with correction at 570 nm